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Agarose gel

An agarose gel is commonly used for electrophoresis of DNA (100bp -60kbp) to segregation and/or purification.

Concentration of agarose vs. length of DNA

agarose conc. % (w/v) DNA (kbp) in TAE DNA (kbp) in TBE
0.3 5.0 - 60
0.6 1.0 - 20 0.9 - 20
0.8 0.8 - 10 0.5 - 9.0
1.0 0.4 - 8.0 0.3 - 7.0
1.2 0.4 - 6.0 0.2 - 5.0
1.5 0.2 - 3.0 0.2 - 4.0
2.0 0.1 - 2.0 0.1 - 3.0

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