Buffers for restriction enzymes

Restriction enzymes perform their function under the suitable conditions. In particular, the temperature and the compositions of the conditions are important. Vendors supply various buffers, some buffers are identical, but the others are different.

Similar buffers

Chart

Low-salt buffers

Takara	L	10mM Tris-HCl	7.5	none	10mM MgCl₂	1mM DTT	-
Nippon gene	L	10mM Tris-HCl	7.5	none	10mM MgCl₂	1mM DTT	-
NEB	1	10mM Bis-Tris-HCl	7.0	none	10mM MgCl₂	1mM DTT	-
Fermentas	B	10mM Tris-HCl	7.5	none	10mM MgCl₂	-	0.1mg/mL BSA
Invitrogen	REact1	50mM Tris-HCl	8.0	none	10mM MgCl₂	-	-
Promega	A	6mM Tris-HCl	7.5	6mM NaCl	6mM MgCl₂	1mM DTT	-

Mid-salt buffers

Takara	M	10mM Tris-HCl	7.5	50mM NaCl	10mM MgCl₂	1mM DTT	-
Nippon gene	M	10mM Tris-HCl	7.5	50mM NaCl	10mM MgCl₂	1mM DTT	-
NEB	2	10mM Tris-HCl	7.9	50mM NaCl	10mM MgCl₂	1mM DTT	-
Fermentas	G	10mM Tris-HCl	7.5	50mM NaCl	10mM MgCl₂	-	0.1mg/mL BSA
Invitrogen	REact2	50mM Tris-HCl	8.0	50mM NaCl	10mM MgCl₂	-	-
Promega	C	10mM Tris-HCl	7.9	50mM NaCl	10mM MgCl₂	1mM DTT	-

High-salt buffers

Takara	H	50mM Tris-HCl	7.5	100mM NaCl	10mM MgCl₂	1mM DTT	-
Nippon gene	H	50mM Tris-HCl	7.5	100mM NaCl	10mM MgCl₂	1mM DTT	-
NEB	3	50mM Tris-HCl	7.9	100mM NaCl	10mM MgCl₂	1mM DTT	-
Fermentas	O	50mM Tris-HCl	7.5	100mM NaCl	10mM MgCl₂	-	0.1mg/mL BSA
Invitrogen	REact3	50mM Tris-HCl	8.0	100mM NaCl	10mM MgCl₂	-	-
Promega	E	6mM Tris-HCl	7.5	100mM NaCl	6mM MgCl₂	1mM DTT	-

KCl buffers

Takara	K	20mM Tris-HCl	8.5	100mM KCl	10mM MgCl₂	1mM DTT	-
Fermentas	R	10mM Tris-HCl	8.5	100mM KCl	10mM MgCl₂	-	0.1mg/mL BSA
Invitrogen	6	50mM Tris-HCl	7.4	50mM KCl/50mM NaCl	6mM MgCl₂	-	-
Promega	J	10mM Tris-HCl	7.5	50mM KCl	7mM MgCl₂	1mM DTT	-

Acetic acid buffers

Takara	T	33mM Tris-OAc	7.9	66mM KOAc	10mM MgOAc₂	0.5mM DTT	-
Nippon gene	A	20mM Tris-OAc	7.9	50mM KOAc₂	10mM MgOAc	1mM DTT	-
NEB	4	20mM Tris-OAc	7.9	50mM KOAc	10mM MgOAc₂	1mM DTT	-
Fermentas	Y	33mM Tris-OAc	7.9	66mM KOAc	10mM MgOAc₂	-	0.1mg/mL

Buffer list of vendors

Takara

Name	Property	buffer	pH	salt	metal ion	reducer
L	Low salt	10mM Tris-HCl	7.5	none	10mM MgCl₂	1mM DTT
M	Mid salt	10mM Tris-HCl	7.5	50mM NaCl	10mM MgCl₂	1mM DTT
H	High salt	50mM Tris-HCl	7.5	100mM NaCl	10mM MgCl₂	1mM DTT
K	KCl salt	20mM Tris-HCl	8.5	100mM KCl	10mM MgCl₂	1mM DTT
T	TrisOAC	33mM Tris-OAc	7.9	66mM KOAc	10mM MgOAc₂	0.5mM DTT

Nippon gene

Name	Property	buffer	pH	salt	metal ion	reducer
L	Low salt	10mM Tris-HCl	7.5	none	10mM MgCl₂	1mM DTT
M	Mid salt	10mM Tris-HCl	7.5	50mM NaCl	10mM MgCl₂	1mM DTT
H	High salt	50mM Tris-HCl	7.5	100mM NaCl	10mM MgCl₂	1mM DTT
A	Aceticacid	20mM Tris-OAc	7.9	50mM KOAc	10mM MgOAc₂	1mM DTT
B	Basic pH	10mM Tris-HCl	8.5	100mM NaCl	10mM MgCl₂	1mM DTT

NEB

Name	Property	buffer	pH	salt	metal ion	reducer
1	low salt	10mM Bis-Tris-HCl	7.0	none	10mM MgCl₂	1mM DTT
2	mid salt	10mM Tris-HCl	7.9	50mM NaCl	10mM MgCl₂	1mM DTT
3	high salt	50mM Tris-HCl	7.9	100mM NaCl	10mM MgCl₂	1mM DTT
4	aceticacid	20mM Tris-OAc	7.9	50mM KOAc	10mM MgOAc₂	1mM DTT

Fermentas

Name	Property	buffer	pH	salt	metal ion	reducer	other
B	Blue	10mM Tris-HCl	7.5	none	10mM MgCl₂	-	0.1mg/mL BSA
G	Green	10mM Tris-HCl	7.5	50mM NaCl	10mM MgCl₂	-	0.1mg/mL BSA
O	Orange	50mM Tris-HCl	7.5	100mM NaCl	10mM MgCl₂	-	0.1mg/mL BSA
R	Red	10mM Tris-HCl	8.5	100mM KCl	10mM MgCl₂	-	0.1mg/mL BSA
Y	Yellow	33mM Tris-OAc	7.9	66mM KOAc	10mM MgOAc₂	-	0.1mg/mL

Invitrogen

Name	Property	buffer	pH	salt	metal ion	reducer	other
1	Low salt	50mM Tris-HCl	8.0	-	10mM MgCl₂	-	-
2	Mid salt	50mM Tris-HCl	8.0	50mM NaCl	10mM MgCl₂	-	-
3	High salt	50mM Tris-HCl	8.0	100mM NaCl	10mM MgCl₂	-	-
4	KCl	20mM Tris-HCl	7.4	50mM KCl	5mM MgCl₂	-	-
6	KCl/NaCl	50mM Tris-HCl	7.4	50mM KCl/50mM NaCl	6mM MgCl₂	-	-
7	KCl/NaCl	50mM Tris-HCl	8.0	50mM KCl/50mM NaCl	10mM MgCl₂	-	-
8	Low salt	20mM Tris-HCl	7.4	-	10mM MgCl₂	-	-
9	High salt	100mM Tris-HCl	7.6	150mM NaCl	10mM MgCl₂	-	-

(*)10mM DTT seperately supplied.

Promega

Name	Property	buffer at 37ºC	pH	salt	metal ion	reducer	other
A	Low salt	6mM Tris-HCl	7.5	6mM NaCl	6mM MgCl₂	1mM DTT	-
B	Mid salt	6mM Tris-HCl	7.5	50mM NaCl	6mM MgCl₂	1mM DTT	-
C	Mid salt	10mM Tris-HCl	7.9	50mM NaCl	10mM MgCl₂	1mM DTT	-
D	High salt	6mM Tris-HCl	7.9	150mM NaCl	6mM MgCl₂	1mM DTT	-
E	High salt	6mM Tris-HCl	7.5	100mM NaCl	6mM MgCl₂	1mM DTT	-
F	High salt	10mM Tris-HCl	8.5	100mM NaCl	10mM MgCl₂	1mM DTT	-
G	Base pH	50mM Tris-HCl	8.2	-	5mM MgCl₂	-	-
H	Strong buffering	90mM Tris-HCl	7.5	50mM NaCl	10mM MgCl₂	-	-
J	Mid KCl	10mM Tris-HCl	7.5	50mM KCl	7mM MgCl₂	1mM DTT	-
K	High KCl	10mM Tris-HCl	7.4	150mM KCl	10mM MgCl₂	-	-
L	Base/High salt	10mM Tris-HCl	9.0	100mM NaCl	3mM MgCl₂	-	-

Links

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For documents available from this page, the author does not warrant or assume any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed.

Buffer, Restriction Enzyme

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