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en:buffer_for_restriction_enzymes [2013/06/09 10:10] (current)
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 +
 +<select Language>​
 +bio:​制限酵素のバッファー|Japanese
 +bio:​en:​Buffer for restriction enzymes|English
 +</​select>​
 +
 +======Buffers for restriction enzymes======
 +Restriction enzymes perform their function under the suitable conditions. ​ In particular, the temperature and the compositions of the conditions are important. Vendors supply various buffers, some buffers ​ are identical, but the others are different.
 +
 +=====Similar buffers=====
 +
 +====Chart====
 +{{bio:​buffer_chart.png|Buffer chart}}
 +
 +====Low-salt buffers====
 +|[[#​Takara]]|L|10mM Tris-HCl|7.5|none|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|[[#Nippon gene]]|L|10mM Tris-HCl|7.5|none|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|[[#​NEB]]|1|10mM Bis-Tris-HCl|7.0|none|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|[[#​Fermentas]]|B|10mM Tris-HCl|7.5|none|10mM MgCl<​sub>​2</​sub>​|-|0.1mg/​mL BSA|
 +|[[#​Invitrogen]]|REact1|50mM Tris-HCl|8.0|none|10mM MgCl<​sub>​2</​sub>​|-|-|
 +|[[#​Promega]]|A|6mM Tris-HCl|7.5|6mM NaCl|6mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +====Mid-salt buffers====
 +|[[#​Takara]]|M|10mM Tris-HCl|7.5|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|[[#Nippon gene]]|M|10mM Tris-HCl|7.5|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|[[#​NEB]]|2|10mM Tris-HCl|7.9|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|[[#​Fermentas]]|G|10mM Tris-HCl|7.5|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|-|0.1mg/​mL BSA|
 +|[[#​Invitrogen]]|REact2|50mM Tris-HCl|8.0|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|-|-|
 +|[[#​Promega]]|C|10mM Tris-HCl|7.9|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +====High-salt buffers====
 +|[[#​Takara]]|H|50mM Tris-HCl|7.5|100mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|[[#Nippon gene]]|H|50mM Tris-HCl|7.5|100mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|[[#​NEB]]|3|50mM Tris-HCl|7.9|100mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|[[#​Fermentas]]|O|50mM Tris-HCl|7.5|100mM NaCl|10mM MgCl<​sub>​2</​sub>​|-|0.1mg/​mL BSA|
 +|[[#​Invitrogen]]|REact3|50mM Tris-HCl|8.0|100mM NaCl|10mM MgCl<​sub>​2</​sub>​|-|-|
 +|[[#​Promega]]|E|6mM Tris-HCl|7.5|100mM NaCl|6mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +====KCl buffers====
 +|[[#​Takara]]|K|20mM Tris-HCl|8.5|100mM KCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|[[#​Fermentas]]|R|10mM Tris-HCl|8.5|100mM KCl|10mM MgCl<​sub>​2</​sub>​|-|0.1mg/​mL BSA|
 +|[[#​Invitrogen]]|6|50mM Tris-HCl|7.4|50mM KCl/50mM NaCl|6mM MgCl<​sub>​2</​sub>​|-|-|
 +|[[#​Promega]]|J|10mM Tris-HCl|7.5|50mM KCl|7mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +
 +====Acetic acid buffers====
 +|[[#​Takara]]|T|33mM Tris-OAc|7.9|66mM KOAc|10mM MgOAc<​sub>​2</​sub>​|0.5mM DTT|-|
 +|[[#Nippon gene]]|A|20mM Tris-OAc|7.9|50mM KOAc<​sub>​2</​sub>​|10mM MgOAc|1mM DTT|-|
 +|[[#​NEB]]|4|20mM Tris-OAc|7.9|50mM KOAc|10mM MgOAc<​sub>​2</​sub>​|1mM DTT|-|
 +|[[#​Fermentas]]|Y|33mM Tris-OAc|7.9|66mM KOAc|10mM MgOAc<​sub>​2</​sub>​|-|0.1mg/​mL|
 +
 +=====Buffer list of vendors=====
 +====Takara====
 +|Name|Property|buffer|pH|salt|metal ion|reducer|
 +|L|Low salt|10mM Tris-HCl|7.5|none|10mM MgCl<​sub>​2</​sub>​|1mM DTT|
 +|M|Mid salt|10mM Tris-HCl|7.5|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|
 +|H|High salt|50mM Tris-HCl|7.5|100mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|
 +|K|KCl salt|20mM Tris-HCl|8.5|100mM KCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|
 +|T|TrisOAC|33mM Tris-OAc|7.9|66mM KOAc|10mM MgOAc<​sub>​2</​sub>​|0.5mM DTT|
 +====Nippon gene====
 +|Name|Property|buffer|pH|salt|metal ion|reducer|
 +|L|Low salt|10mM Tris-HCl|7.5|none|10mM MgCl<​sub>​2</​sub>​|1mM DTT|
 +|M|Mid salt|10mM Tris-HCl|7.5|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|
 +|H|High salt|50mM Tris-HCl|7.5|100mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|
 +|A|Aceticacid|20mM Tris-OAc|7.9|50mM KOAc|10mM MgOAc<​sub>​2</​sub>​|1mM DTT|
 +|B|Basic pH|10mM Tris-HCl|8.5|100mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|
 +====NEB====
 +|Name|Property|buffer|pH|salt|metal ion|reducer|
 +|1|low salt|10mM Bis-Tris-HCl|7.0|none|10mM MgCl<​sub>​2</​sub>​|1mM DTT|
 +|2|mid salt|10mM Tris-HCl|7.9|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|
 +|3|high salt|50mM Tris-HCl|7.9|100mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|
 +|4|aceticacid|20mM Tris-OAc|7.9|50mM KOAc|10mM MgOAc<​sub>​2</​sub>​|1mM DTT|
 +====Fermentas====
 +|Name|Property|buffer|pH|salt|metal ion|reducer|other|
 +|B|Blue|10mM Tris-HCl|7.5|none|10mM MgCl<​sub>​2</​sub>​|-|0.1mg/​mL BSA|
 +|G|Green|10mM Tris-HCl|7.5|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|-|0.1mg/​mL BSA|
 +|O|Orange|50mM Tris-HCl|7.5|100mM NaCl|10mM MgCl<​sub>​2</​sub>​|-|0.1mg/​mL BSA|
 +|R|Red|10mM Tris-HCl|8.5|100mM KCl|10mM MgCl<​sub>​2</​sub>​|-|0.1mg/​mL BSA|
 +|Y|Yellow|33mM Tris-OAc|7.9|66mM KOAc|10mM MgOAc<​sub>​2</​sub>​|-|0.1mg/​mL|
 +====Invitrogen====
 +|Name|Property|buffer|pH|salt|metal ion|reducer|other|
 +|1|Low salt|50mM Tris-HCl|8.0|-|10mM MgCl<​sub>​2</​sub>​|-|-|
 +|2|Mid salt|50mM Tris-HCl|8.0|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|-|-|
 +|3|High salt|50mM Tris-HCl|8.0|100mM NaCl|10mM MgCl<​sub>​2</​sub>​|-|-|
 +|4|KCl|20mM Tris-HCl|7.4|50mM KCl|5mM MgCl<​sub>​2</​sub>​|-|-|
 +|6|KCl/​NaCl|50mM Tris-HCl|7.4|50mM KCl/50mM NaCl|6mM MgCl<​sub>​2</​sub>​|-|-|
 +|7|KCl/​NaCl|50mM Tris-HCl|8.0|50mM KCl/50mM NaCl|10mM MgCl<​sub>​2</​sub>​|-|-|
 +|8|Low salt|20mM Tris-HCl|7.4|-|10mM MgCl<​sub>​2</​sub>​|-|-|
 +|9|High salt|100mM Tris-HCl|7.6|150mM NaCl|10mM MgCl<​sub>​2</​sub>​|-|-|
 +(*)10mM DTT seperately supplied.
 +====Promega====
 +|Name|Property|buffer at 37&​ordm;​C|pH|salt|metal ion|reducer|other|
 +|A|Low salt|6mM Tris-HCl|7.5|6mM NaCl|6mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|B|Mid salt|6mM Tris-HCl|7.5|50mM NaCl|6mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|C|Mid salt|10mM Tris-HCl|7.9|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|D|High salt|6mM Tris-HCl|7.9|150mM NaCl|6mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|E|High salt|6mM Tris-HCl|7.5|100mM NaCl|6mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|F|High salt|10mM Tris-HCl|8.5|100mM NaCl|10mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|G|Base pH|50mM Tris-HCl|8.2|-|5mM MgCl<​sub>​2</​sub>​|-|-|
 +|H|Strong buffering|90mM Tris-HCl|7.5|50mM NaCl|10mM MgCl<​sub>​2</​sub>​|-|-|
 +|J|Mid KCl|10mM Tris-HCl|7.5|50mM KCl|7mM MgCl<​sub>​2</​sub>​|1mM DTT|-|
 +|K|High KCl|10mM Tris-HCl|7.4|150mM KCl|10mM MgCl<​sub>​2</​sub>​|-|-| ​
 +|L|Base/​High salt|10mM Tris-HCl|9.0|100mM NaCl|3mM MgCl<​sub>​2</​sub>​|-|-|
 +======Links======
 +
 +  *[[Restriction enzyme]]
 +  *[[Restriction-Modification system]]
 +  *[[Buffer solution|Buffer solution (Good'​s buffer etc.)]]
 +  *[[http://​bio.takara.co.jp/​BIO_EN/​catalog_d.asp?​C_ID=C0003|Restriction enzyme activity displaying system using universal and basal buffers (TaKaRa BIO)]]
 +  *[[http://​nippongene.com/​pages/​products/​clomod/​res_e/​reinfo01.html|ニッポンジーンの制限酵素について(ニッポンジーン)]]
 +  *[[http://​www.neb.com/​nebecomm/​products/​category63.asp#​71|Buffers & Supplements (NEB)]]
 +  *[[http://​www.fermentas.com/​techinfo/​re/​5bufferplussystem.htm|Five Buffer System (Fermentas AB)]]
 +  *[[http://​www.invitrogen.com/​content/​sfs/​manuals/​complete_react_buffer_set_man.pdf|REact buffer set table(PDF 360KB)]]
 +  *[[http://​www.promega.com/​guides/​re_guide/​chapthree/​table3_3.htm|Buffer compositions]]
 +
 +
 +
 +
 +======Disclaimer======
 +For documents available from this page, the author does not warrant or assume any legal liability or responsibility for the accuracy, completeness,​ or usefulness of any information,​ apparatus, product, or process disclosed.
 +
 +
 +{{tag>​en:​Buffer en:​Restriction_Enzyme}}