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Chloramphenicol

Chloramphenicol is an antibiotic that inhibits translation of protein from mRNA. It binds A site in a ribosome 50S subunit and inhibits peptidyltransferase activity.

PubChem image 5959

Resistance

Preparation

  1. Solve chloramphenicol 1.5g in methanol and adjust to 50mL.
  2. Dispense into 1ml vials and store at -20°C.
  3. Add 1mL (1vial) in the 1L medium.
Stock conc.Using conc.
30mg/mL30µg/mL

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