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en:chloramphenicol [2013/06/09 10:10] (current)
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 +======Chloramphenicol======
 +Chloramphenicol is an antibiotic that inhibits translation of protein from mRNA. It binds A site in a ribosome 50S subunit and inhibits peptidyltransferase activity. ​
 +
 +{{pubchem>​l_template:​5959|Chloramphenicol,​ Chloromycetin}}
 +
 +=====Resistance=====
 +  *[[Chloramphenicol acetyltransferase]]
 +    *[[interpro>​IPR001707]]
 +
 +=====Preparation=====
 +
 +  -Solve chloramphenicol 1.5g in methanol and adjust to 50mL.
 +  -Dispense into 1ml vials and store at -20°C.
 +  -Add 1mL (1vial) in the 1L medium.
 +
 +^Stock conc.^Using conc.^
 +|30mg/​mL|30µg/​mL|
 +
 +
 +
 +======Links======
 +
 +  *[[Antibiotics]]
 +  *[[Chloramphenicol acetyltransferase]]
 +  *MeSH: [[MeSH>​68002701]]
 +
 +
 +{{tag>​antibiotics preparation}}