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en:chloramphenicol_acetyltransferase [2013/06/09 10:09] (current)
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 +======Chloramphenicol acetyltransferase======
 +Chloramphenicol acetyltransferase acetylate the hydroxyl groups of [[Chloramphenicol|chloramphenicol (Cm)]] from the acetyl-CoA. The acetylated Cm cannot bind ribosome 50S and is inactive.
 +
 +=====InterPro entry=====
 + -> [[interpro>​IPR001451]]
 +
 +Chloramphenicol acetyltransferase (CAT) (EC:​2.3.1.28) [1] catalyzes the acetyl-CoA dependent acetylation of chloramphenicol (Cm), an antibiotic which inhibits prokaryotic peptidyltransferase activity. Acetylation of Cm by CAT inactivates the antibiotic. A histidine residue, located in the C-terminal section of the enzyme, plays a central role in its catalytic mechanism.
 +
 +There is a second family of CAT [2], evolutionary unrelated to the main family described above. These CAT belong to the bacterial hexapeptide-repeat containing-transferases family ​
 +
 +The crystal structure of the type III enzyme from Escherichia coli with chloramphenicol bound has been determined. CAT is a trimer of identical subunits (monomer Mr 25,000) and the trimeric structure is stabilized by a number of hydrogen bonds, some of which result in the extension of a beta-sheet across the subunit interface. Chloramphenicol binds in a deep pocket located at the boundary between adjacent subunits of the trimer, such that the majority of residues forming the binding pocket belong to one subunit while the catalytically essential histidine belongs to the adjacent subunit. His195 is appropriately positioned to act as a general base catalyst in the reaction, and the required tautomeric stabilization is provided by an unusual interaction with a main-chain carbonyl oxygen [3].
 +
 +======PDB======
 +
 +{{pdb>​fullSmall:​3cla}}
 +{{pdb>​fullSmall:​1cia}}
 +
 +{{pdb>​link:​1cla}}, ​
 +{{pdb>​link:​1noc}}, ​
 +{{pdb>​link:​1pd5}}, ​
 +{{pdb>​link:​1q23}}, ​
 +{{pdb>​link:​1qca}}, ​
 +{{pdb>​link:​2cla}}, ​
 +{{pdb>​link:​4cla}}
 +
 +
 +======Reference======
 +
 +  -{{pubmed>​long:​9334294}}
 +  -{{pubmed>​long:​7500366}}
 +  -{{pubmed>​long:​1867721}}
 +
 +
 +======Links======
 +
 +  *[[Antibiotics]]
 +  *[[Chloramphenicol]]
 +
 +
 +{{tag>​Enzyme}}