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en:comparison_between_tae_and_tbe [2013/06/09 10:10] (current)
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 +======Comparison between TAE and TBE======
 +There are some differences between TAE buffer and TBE buffer. Generally, 1xTAE or 0.5xTAE is used for agarose gel electrophoresis,​ whereas 1xTBE is used for PAGE.
 +
 +======Comparison======
 +=====Recovery of nucleic acids=====
 +TAE has been prefavorably used so far because of the poor recovery of nucleic acids from TBE-gel. However, recent commercial recovery kits utilizing silica works well for either TAE-gel or TBE-gel.
 +
 +
 +=====Cost=====
 +
 +^ ^TAE^TBE^note^
 +^Recovery|O|Δ|depending on\\  the method|
 +^Price|O|X|TAE<​TBE|
 +^Moving rate|X|O|Slow rate\\ -->Long G.E.\\ --->High temp.|
 +^Buffering|Δ|O|TAE<​TBE|
 +
 +
 +TAE is cheaper than TBE. Price of TAE is 40% lower than TBE at maximum according to volume.
 +Concentration of stock solution of TAE and TBE are 50x and 10x, respectively. So, many TBE bottles occupies more space than TAE does.
 +
 +=====Moving rate=====
 +Since ion strength of TBE is higher than TAE, solutes in TBE move faster than in TAE.
 +Moreover, temperature of gel increases using TAE-gel for a long time, pH might significantly decrease because of [[Tris]]'​s temperature dependency of pKa. Fluorescent moiety of labelled DNA might go bad in the conditions of high temperature and lowered pH.
 +
 +=====Buffering capacity=====
 +Buffering capacity of TBE is greater than that of TAE.
 +
 +======Links======
 +
 +  *[[TAE buffer]]
 +  *[[TBE buffer]]
 +
 +
 +
 +{{tag>​Buffer}}