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en:dithiothreitol [2013/06/09 10:10] (current)
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 +======Dithiothreitol======
 +A reagent commonly used in biochemical studies as a protective agent to prevent the oxidation of SH (thiol) groups and for reducing disulphides to dithiols. Less odorant reagent than other organic chemicals containing thiol groups. (MeSH: [[MeSH>​68004229]])
 +
 +{{pubchem>​l_template:​19001|Dithiothreitol}}
 +
 +=====Preparation=====
 +
 +**0.5 M DTT aq.**\\ ​
 +  *Disolve 3.9 g of DTT in <​chem>​H2O</​chem>​.
 +  *Add <​chem>​H2O</​chem>​ upto 50 mL.
 +
 +
 +
 +=====Redox reaction of DTT=====
 +
 +**Reduced**\\ ​
 +{{pubchem>​small:​19001}}
 +
 +
 +\\ \\ 
 +{{icon>​arrows_large}}\\ ​
 +<​chem>​2H+,​ 2e-</​chem>​
 +
 +
 +**Oxidized**\\ ​
 +{{pubchem>​small:​439407}}
 +
 +
 +
 +{{tag>​redox}}