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en:dpni [2013/06/09 10:10]
en:dpni [2013/06/09 10:10] (current)
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 +======DpnI======
 +DpnI cuts sites '​GATC'​ if the adenine is methylated.
 +
 +^  Recognition ​ |  {{:​re_g.ma-tc.png|GATC}} ​  |||||
 +^{{icon>​therm}}Temp.| ​ 37 ºC  |||||
 +^{{icon>​microtube}}Buffer^[[buffer for restriction enzymes#​Low-salt_buffers|Low]]^[[buffer for restriction enzymes#​Mid-salt_buffers|Mid]]^[[buffer for restriction enzymes#​High-salt_buffers|High]]^[[buffer for restriction enzymes#​Acetic acid buffers|Ac]]^[[buffer for restriction enzymes#KCl buffers|KCl]]^ ​
 +^  ≥75% ​ |  X  |  X  |  X  |  O  |  X  |
 +
 +=====Reaction=====
 +DpnI cuts sites '​GmATC'​ (mA: N6-methyladenine). ​ The structures below are part of dam-methylated pUC19 and non-methylated pUC19, respectively.
 +
 +^Methylated - reactive^Unmethylated - inert^
 +|  {{bio:​dpni_reactive.png|Methylated}} ​ |  {{bio:​dpni_inert.png|Unmethylated}} ​ |
 +
 +=====Comparison with DpnII and MboI=====
 +DpnII and MboI are isoscizomers that recognize a same sequence and cut at same position. These REases can cut unmethylated sequence.
 +^Methylated - inert^Unmethylated - reactive^
 +|  {{bio:​dpnii_inert.png|Methylated}} ​ |  {{bio:​dpnii_reactive.png|Unmethylated}} ​ |
 +
 +======Links======
 +
 +  *[[Restriction enzyme]]
 +    *[[DpnII]] {{icon>​sticky5}}
 +    *[[Sau3AI]] {{icon>​sticky5}}
 +    *[[MboI]] {{icon>​sticky5}}
 +    *[[Buffer for restriction enzymes]]
 +  *[[http://​rebase.neb.com/​rebase/​rebase.html|REBASE]]
 +
 +
 +{{tag>​Restriction_enzyme blunt}}