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en:edetic_acid [2013/06/09 10:09] (current)
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 +======Edetic acid======
 +{{pubchem>​l_template:​6049|Edetic acid, EDTA}}
 +
 +=====Preparation=====
 +====0.5M EDTA (pH 8.0)====
 +
 +{{pubchem>​s_template:​636371|2Na-EDTA dihydrate}}
 +
 +\\ \\ 
 +^[[Disodium edetate|EDTA-2Na]]-2<​chem>​H2O</​chem>​ |186.1 g|
 +^<​chem>​H2O</​chem>​|700 mL (final 1L)|
 +
 +  -Solve EDTA by adding NaOH pelette, and adjust pH to 8.0.
 +  -Add <​chem>​H2O</​chem>​ to 1 L.
 +  -Autoclave.
 +
 +If 1L of EDTA stock solution is too much, scale down according to your experiments.
 +
 +
 +======Links======
 +
 +  *[[TE]]
 +
 +
 +{{tag>​preparation buffer}}