Precipitation of polynucleotide such as DNA and RNA with ethanol aqueous solution to purify the polynucleotide.

1. Add 1/10 volume of 3M AcONa.
2. Add 2x volume of cold 100% EtOH.
3. Incubate for 20 min at r.t.
4. Centrifuge at 4 ºC, 15000rpm for 10 - 15 min.
5. Remove EtOH by pippetting or decantation.
6. Add cold 70% EtOH aq. and mix.
7. Centrifuge for 5 - 10 min.
8. If necessary, dry by using centrifuging evaporator.

• To prepare dried DNA.
• To remove excess salt from the solution.
• To purify DNA from the solution after Ph/Chl protein precipitation.