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G418 (Geneticin)

G418 is an inhibitor of protein synthesis.

Molecular Weight 496.553 g/mol
Molecular Formula C20H40N4O10

PubChem image 123865

Resistance

Preparation

G418 is available either solid or aqueous solution (50mg/mL)
The activity of solid G418 decreases to 70% activity
(See Invitrogen's brochure.

Solution conc.Using conc.per 1Lper 100mL
50mg/mL300-1100μg/mL600 - 2200 μL60 - 220μL

50mL solution (weight)
= (concentration)x(volume)÷(relative activity)
= 50 x 0.05 ÷ 0.7 = 3.57 g

20mL solution (weight)
= (concentration)x(volume)÷(relative activity)
= 50 x 0.02 ÷ 0.7 = 1.43 g

  1. Solve G418 sulfate in H2O and adjust to 50mL or 20mL.
  2. Sterilize using filter and store at 4°C or -20°C.

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