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en:g418 [2013/06/09 10:10] (current)
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 +
 +<select Language>​
 +bio:​G418|日本語
 +bio:​en:​G418|English
 +</​select>​
 +
 +======G418 (Geneticin)======
 +G418 is an inhibitor of protein synthesis. ​
 +
 +
 +^Molecular Weight| ​ 496.553 g/mol  |
 +^Molecular Formula| <​chem>​C20H40N4O10</​chem>​ |
 +
 +
 +{{pubchem>​large:​123865}}
 +
 +
 +=====Resistance=====
 +  *[[Aminoglycoside phosphotransferase|Neor/​Kanr (Aminoglycoside phosphotransferase)]]
 +    *InterPro entry {{interpro>​link:​IPR012149}}
 +=====Preparation=====
 +
 +G418 is available either solid or aqueous solution (50mg/​mL)\\ ​
 +The activity of solid G418 decreases to 70% activity\\
 + (See [[http://​www.invitrogen.com/​content/​sfs/​manuals/​3756.pdf|Invitrogen'​s brochure]].\\ \\ 
 +^Solution conc.^Using conc.^per 1L^per 100mL^
 +|50mg/​mL|300-1100μg/​mL|600 - 2200 μL|60 - 220μL|
 +
 +
 +
 +**50mL solution**
 +(weight)\\ ​
 + = (concentration)x(volume)÷(relative activity)\\ ​
 + = 50 x 0.05 ÷ 0.7 = **3.57 g**
 +
 +**20mL solution**
 +(weight)\\ ​
 + = (concentration)x(volume)÷(relative activity)\\ ​
 + = 50 x 0.02 ÷ 0.7 = **1.43 g**
 +
 +  -Solve [[G418 sulfate]] in <​chem>​H2O</​chem>​ and adjust to 50mL or 20mL.
 +  -[[filter sterilization|Sterilize using filter]] and store at 4°C or -20°C.
 +
 +======Links======
 +
 +  *[[Antibiotics]]
 +    *[[Kanamycin]]
 +    *[[Neomycin]]
 +
 +
 +{{tag>​antibiotics preparation}}