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en:hepes [2013/06/09 10:09] (current)
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 +======HEPES======
 +HEPES is one of the [[buffer_solution|Goods'​ buffer]]s.
 +
 +^Name| HEPES((2-[4-(2-Hydroxyethyl)-1-piperazinyl] ethanesulfonic acid)) |
 +^Molecular Formula| <​chem>​C8H18N2O4S</​chem>​ |
 +^Molecular Weight| 238.306|
 +^CAS No.| 7365-45-9 |
 +^pKa(25ºC)| 7.48 |
 +
 +{{pubchem>​large:​23831}}
 +
 +=====Preparation=====
 +====1M HEPES-KOH====
 +
 +  -Disolve HEPES(238.3 g) in 800 ml <​chem>​H2O</​chem>​.
 +  -Adjust pH by addition of KOH.
 +  -Add <​chem>​H2O</​chem>​ upto 1 L.
 +  -If neccessary, autoclave.
 +  -Store at 4°C.
 +
 +
 +{{tag>​buffer preparation}}