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en:kanamycin [2013/06/09 10:10] (current)
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 +
 +<select Language>​
 +bio:​カナマイシン|日本語
 +bio:​en:​kanamycin|English
 +</​select>​
 +
 +======Kanamycin======
 +Kanamycin is an antibiotics that inhibits synthesis of protein by binding to 30S ribosome. ​
 +
 +
 +^Name|Kanamycin(( (2R,​3S,​4S,​5R,​6R)-2-(aminomethyl)-6-[(1R,​2R,​3S,​4R,​6S)-4,​6-diamino-3-[(2S,​3R,​
 +4S,​5S,​6R)-4-amino-3,​5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2-hydroxycyc
 +lohexyl]oxyoxane-3,​4,​5-triol ))|
 +^MeSH| [[MeSH>​68007612]] |
 +^Molecular Weight| ​ 484.499 g/mol  |
 +^Molecular Formula| <​chem>​C18H36N4O11</​chem>​ |
 +
 +
 +**Kanamycin A**\\ 
 +{{pubchem>​large:​6032}}
 +
 +
 +
 +=====Resistance=====
 +  *[[Aminoglycoside phosphotransferase|Neor/​Kanr (Aminoglycoside phosphotransferase)]]
 +  *[[interpro>​IPR012149|InterPro entry]]
 +
 +=====Preparation=====
 +
 +  -Solve [[Kanamycin sulfate]] 5g in <​chem>​H2O</​chem>​ or 20%EtOH aq.
 +  -[[Filter sterilization|Sterilize using filter]] and dispense into 1mL vials.
 +  -Store at -20°C.
 +  -On using, add 1mL (1 vial) into 1L medium.
 +
 +^Stock conc.^Using conc.^
 +|100mg/​mL|100µg/​mL|
 +
 +
 +
 +======Links======
 +
 +  *[[Antibiotics]]
 +    *[[Neomycin]]
 +    *[[G418]]
 +
 +
 +{{tag>​antibiotics preparation}}