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en:mboi [2013/06/09 10:09] (current)
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 +======MboI======
 +^  Recognition ​ |  {{:​re_gatc.png|GATC}} ​  |||||
 +^{{icon>​therm}}Temp.| ​ 37 ºC  |||||
 +^{{icon>​microtube}}Buffer^[[buffer for restriction enzymes#​Low-salt_buffers|Low]]^[[buffer for restriction enzymes#​Mid-salt_buffers|Mid]]^[[buffer for restriction enzymes#​High-salt_buffers|High]]^[[buffer for restriction enzymes#​Acetic acid buffers|Ac]]^[[buffer for restriction enzymes#KCl buffers|KCl]]^ ​
 +^  ≥75% ​ |  X  |  O  |  O  |  O  |  O  | 
 +
 +=====Isoscizomer=====
 +====Sau3AI====
 +[[Sau3AI]] is an isoscizomer of MboI.  Sau3AI can cut methylated sites, so it is often used for partial digestion of genome DNA.
 +
 +======Links======
 +
 +  *[[Restriction enzyme]]
 +    *[[Sau3AI]] {{icon>​sticky5}}
 +    *[[DpnI]] ​ {{icon>​blunt}}
 +    *[[DpnII]] {{icon>​sticky5}}
 +    *[[Buffer for restriction enzymes]]
 +  *[[http://​rebase.neb.com/​rebase/​rebase.html|REBASE]]
 +
 +
 +{{tag>​Restriction_enzyme 5'​sticky}}