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en:methylase [2013/01/31 05:38] (current)
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 +======Methylase======
 +(Methylase, Methyltransferase,​ MTase)
 +
 +Enzyme in the [[restriction-modification system]] of bacteria that transfer methyl group (or hydroxymethyl) to cytosine or adenine. TypeII methylases are usually used for construction or cloning.
 +
 +|M.EcoRI||
 +|Recognition|Methylated|
 +|{{:​me_ecori_reactive.png|}}|{{:​me_ecori_inert.png|}}|
 +
 +Adenines are methylated by M.EcoRI in this example.
 +
 +Sometimes methyl groups on the DNA prevent from digestion by restriction enzyme. ​ Since bacteria used for cloning often dam methylase and dcm methylase, it is neccessary to pay attention to the methylated sites.
 +