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Protruding region of dsDNA or the sequence of the protruding region.

Biological application

If over-hang of termini of a cloning vector and a insert are complimentary, they hybridizes and are ligated effectively. The direction of the insert can be controlled by using two differenct over-hang sequences.

How to produce

  • Digest with a restriction enzyme.
  • Add a recognition sequence of a restriction enzyme to 5'-terminus of primers used in PCR
  • Use an over-hang fragment produced with Taq PCR. TdT activity of Taq adds an adenine to end of the fragment (TA-cloning)
  • Anneal ssDNA (or ssRNA).