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Polyacrylamide gel

A polyacrylamide gel is commonly used for electrophoresis of small DNAs (~10 - 2000bp). An agarose gel is also used for electrophoresis for more long DNA strands.

Concentration of acrylamide vs. length of DNA

conc. of acrylamide % (w/v) DNA (bp) Cyanol FF(XC) Bromophenol blue(BPB)
3.5 1000-2000 460 100
5 80 - 500 260 65
8 60 - 400 160 45
12 40 - 200 70 20
15 25 - 150 60 15
20 6 - 100 45 12

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