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en:polyacrylamide_gel [2013/06/09 10:09] (current)
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 +======Polyacrylamide gel======
 +A polyacrylamide gel is commonly used for electrophoresis of small DNAs (~10 - 2000bp). An [[agarose gel]] is also used for electrophoresis for more long DNA strands.
 +
 +=====Concentration of acrylamide vs. length of DNA =====
 +^  conc. of [[acrylamide]] % (w/v)  ^  DNA (bp)  ^  [[Cyanol FF]](XC) ​ ^  [[Bromophenol blue]](BPB) ​ ^
 +^  3.5  |  1000-2000 ​ |  460  |  100  |
 +^    5  |   80 - 500  |  260  |   ​65 ​ |
 +^    8  |   60 - 400  |  160  |   ​45 ​ |
 +^  12   ​| ​  40 - 200  |  70   ​| ​  ​20 ​ |
 +^  15   ​| ​  25 - 150  |  60   ​| ​  ​15 ​ |
 +^  20   ​| ​  6 - 100   ​| ​ 45   ​| ​  ​12 ​ |
 +
 +======Links======
 +
 +  *[[Agarose gel]]
 +  *[[:​ポリアクリルアミドゲル]]
 +
 +
 +{{tag>​DNA purification electrophoresis gel}}