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en:sau3ai [2013/06/09 10:09] (current)
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 +======Sau3AI======
 +^  Recognition ​ |  {{:​re_gatc.png|GATC}} ​  |||||
 +^{{icon>​therm}}Temp.| ​ 37 ºC  |||||
 +^{{icon>​microtube}}Buffer^[[buffer for restriction enzymes#​Low-salt_buffers|Low]]^[[buffer for restriction enzymes#​Mid-salt_buffers|Mid]]^[[buffer for restriction enzymes#​High-salt_buffers|High]]^[[buffer for restriction enzymes#​Acetic acid buffers|Ac]]^[[buffer for restriction enzymes#KCl buffers|KCl]]^ ​
 +^  ≥75% ​ |  O  |  O  |  O  |  O  |  X  | 
 +
 +=====Isoscizomer=====
 +The recognition sites of isoscizomer,​ [[MboI]] will be blocked by dam methylase. On the other hand, Sau3A can cut the GATC sites even if the adenines are methylated. Therefore, Sau3AI is used for mapping or construction of library of methylated genome.
 +
 +======Links======
 +
 +  *[[Restriction enzyme]]
 +    *[[MboI]] {{icon>​sticky5}}
 +    *[[DpnI]] ​ {{icon>​blunt}}
 +    *[[DpnII]] {{icon>​sticky5}}
 +  *[[Buffer for restriction enzymes]]
 +  *[[http://​rebase.neb.com/​rebase/​rebase.html|REBASE]]
 +
 +
 +{{tag>​Restriction_enzyme 5'​sticky}}