Table of Contents

Star activity

It has been demonstrated that under extreme non-standard conditions, restriction endonucleases are capable of cleaving sequences which are similar but not identical to their defined recognition sequence. This altered or relaxed specificity has been termed “star” activity.


  1. Relaxation of recognition sequence of specific endonuclease HindIII.
    Nasri M, Thomas D
    Nucleic Acids Res14p811-21(1986 Jan 24)
  2. Sequence-specific endonuclease BamHI: relaxation of sequence recognition.
    George J, Chirikjian JG
    Proc Natl Acad Sci U S A79p2432-6(1982 Apr)
  3. Relaxed specificity of endonuclease BamH1 as determined by identification or recognition sites in SV 40 and pBR 322 DNAs.
    Kolesnikov VA, Zinoviev VV, Yashina LN, Karginov VA, Baclanov MM, Malygin EG
    FEBS Lett132p101-4(1981 Sep 14)
  4. Specificity of substrate recognition by the EcoRI restriction endonuclease.
    Polisky B, Greene P, Garfin DE, McCarthy BJ, Goodman HM, Boyer HW
    Proc Natl Acad Sci U S A72p3310-4(1975 Sep)
  5. DNA site recognition and reduced specificity of the Eco RI endonuclease.
    Woodbury CP Jr, Hagenbüchle O, von Hippel PH
    J Biol Chem255p11534-48(1980 Dec 10)
  6. Alteration of the specificity of PvuII restriction endonuclease.
    Nasri M, Thomas D
    Nucleic Acids Res15p7677-87(1987 Oct 12)
  7. Self-generated DNA termini relax the specificity of SgrAI restriction endonuclease.
    Bitinaite J, Schildkraut I
    Proc Natl Acad Sci U S A99p1164-9(2002 Feb 5)
  8. Cloning and sequencing of restriction fragments generated by Eco RI*.
    Gardner RC, Howarth AJ, Messing J, Shepherd RJ