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+ | ======Streptomycin====== | ||
+ | An antibiotic produced by the soil actinomycete //[[taxonomy>1911|Streptomyces griseus]]//. It acts by inhibiting the initiation and elongation processes during protein synthesis. ([[MeSH>68013307|MeSH]]) | ||
+ | |||
+ | {{pubchem>l_template:5999|Streptomycin}} | ||
+ | |||
+ | =====Preparation===== | ||
+ | |||
+ | -Solve [[Streptomycin sulfate]] 500mg in <chem>H2O</chem> or 20% EtOH aq. | ||
+ | -Adjust the volume to 50mL. | ||
+ | -[[Filter sterilization|Sterilize using filter]] and dispense into 1mL vials. | ||
+ | -Store at -20ºC. | ||
+ | -Use 1mL (1vial) in a 1L medium. | ||
+ | |||
+ | ^Stock conc.^Using conc.^ | ||
+ | |10mg/mL|10µg/mL| | ||
+ | |||
+ | |||
+ | =====Resistance===== | ||
+ | |||
+ | *Aminoglycoside phosphotransferase - [[interpro>IPR002575]] | ||
+ | *Streptomycin adenyltransferase - [[interpro>IPR007530]] | ||
+ | *[[uniprot>P17585]] - UniProt (knowledgebase) | ||
+ | |||
+ | |||
+ | {{pdb>fullSmall:2pbe}} | ||
+ | |||
+ | ====== References ====== | ||
+ | |||
+ | *{{pubmed>long:9211644}} | ||
+ | *{{pubmed>long:2167474}} | ||
+ | |||
+ | |||
+ | {{tag>Antibiotics Preparation}} | ||