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en:streptomycin [2013/06/09 10:09] (current)
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 +======Streptomycin======
 +An antibiotic produced by the soil actinomycete //​[[taxonomy>​1911|Streptomyces griseus]]//​. It acts by inhibiting the initiation and elongation processes during protein synthesis. ([[MeSH>​68013307|MeSH]])
 +
 +{{pubchem>​l_template:​5999|Streptomycin}}
 +
 +=====Preparation=====
 +
 +  -Solve [[Streptomycin sulfate]] 500mg in <​chem>​H2O</​chem>​ or 20% EtOH aq.
 +  -Adjust the volume to 50mL.
 +  -[[Filter sterilization|Sterilize using filter]] and dispense into 1mL vials.
 +  -Store at -20ºC.
 +  -Use 1mL (1vial) in a 1L medium.
 +
 +^Stock conc.^Using conc.^
 +|10mg/​mL|10µg/​mL|
 +
 +
 +=====Resistance=====
 +
 +  *Aminoglycoside phosphotransferase - [[interpro>​IPR002575]]
 +  *Streptomycin adenyltransferase - [[interpro>​IPR007530]]
 +  *[[uniprot>​P17585]] - UniProt (knowledgebase)
 +
 +
 +{{pdb>​fullSmall:​2pbe}}
 +
 +====== References ======
 +
 +  *{{pubmed>​long:​9211644}}
 +  *{{pubmed>​long:​2167474}}
 +
 +
 +{{tag>​Antibiotics Preparation}}