Differences

This shows you the differences between two versions of the page.

@@ Line 1: / Line 1: @@
+======Streptomycin======
+An antibiotic produced by the soil actinomycete //[[taxonomy>1911|Streptomyces griseus]]//. It acts by inhibiting the initiation and elongation processes during protein synthesis. ([[MeSH>68013307|MeSH]])
+{{pubchem>l_template:5999|Streptomycin}}
+=====Preparation=====
+  -Solve [[Streptomycin sulfate]] 500mg in <chem>H2O</chem> or 20% EtOH aq.
+  -Adjust the volume to 50mL.
+  -[[Filter sterilization|Sterilize using filter]] and dispense into 1mL vials.
+  -Store at -20ºC.
+  -Use 1mL (1vial) in a 1L medium.
+^Stock conc.^Using conc.^
+|10mg/mL|10µg/mL|
+=====Resistance=====
+  *Aminoglycoside phosphotransferase - [[interpro>IPR002575]]
+  *Streptomycin adenyltransferase - [[interpro>IPR007530]]
+  *[[uniprot>P17585]] - UniProt (knowledgebase)
+{{pdb>fullSmall:2pbe}}
+====== References ======
+  *{{pubmed>long:9211644}}
+  *{{pubmed>long:2167474}}
+{{tag>Antibiotics Preparation}}

BioWiki