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T-vector

A single-Thymine protruding fragment. T-vectors are used in TA-cloning.

Preparation

A single-protruding fragment can be produced by changing the substrate of the TdT reaction.

SystemProductReference
Taq + dTTPThymine-protruding1,3
TdT + ddTTPThyimine-protruding2,3

References

  1. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.
    Marchuk D, Drumm M, Saulino A, Collins FS
    Nucleic Acids Res19p1154(1991 Mar 11)
  2. Ming-Yi Zhou and Celso E. Gomez-Sanchez, Universal TA Cloning. Current Issues in Molecular Biology, (2000) 2,1-7.
    (http://www.open-access-biology.com/pcr/pcr-zhou.html)

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