BioWiki

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

en:t-vector [2013/06/09 10:10] (current)
Line 1: Line 1:
 +======T-vector======
 +A single-Thymine protruding fragment. ​ T-vectors are used in TA-cloning.
 +
 +{{bio:​tvector.png}}
 +
 +=====Preparation=====
 +A single-protruding fragment can be produced by changing the substrate of the TdT reaction.
 +^System^Product^Reference^
 +^Taq + dTTP|Thymine-protruding|1,​3|
 +^TdT + ddTTP|Thyimine-protruding|2,​3|
 +
 +======References======
 +
 +  -{{pubmed>​long:​2020552}}
 +  -{{pubmed>​long:​2020554}}
 +  -Ming-Yi Zhou and Celso E. Gomez-Sanchez,​ Universal TA Cloning. //Current Issues in Molecular Biology//, (2000) 2,1-7.\\ ([[http://​www.open-access-biology.com/​pcr/​pcr-zhou.html]])
 +
 +
 +======Link======
 +
 +  *[[TA-cloning]]
 +
 +