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en:t4_dna_polymerase [2013/06/09 10:10] (current)
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 +======T4 DNA polymerase======
 +T4 DNA polymerase has two activities, 5'​->​3'​ polymerase activity and 3'​->​5'​ single-strand specific exonuclease activity. T4 DNA polymerase is often used for to do [[blunting sticky ends]].
 +=====Reaction=====
 +
 +^3'​->​5'​ exonuclease\\ activity^5'​->​3'​polymerase\\ activity^
 +|{{bio:​nuclease_activity_35.png|nuclease activity}}|{{{{bio:​polymerase_activity_53.png|polymerase activity}}|
 +
 +The activities of T4 DNA polymerase are utilized to blunt sticky ends.
 +  -5' sticky end -> filled-in by the polymerase activity.
 +  -3' sticky end -> digested by exonuclease activity.
 +
 +
 +======Links======
 +
 +  *[[Blunting sticky ends]]
 +  *[[Taq DNA Polymerase I]]
 +  *[[Pfu DNA Ligase]]
 +
 +
 +