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en:tae_buffer [2013/06/09 10:09] (current)
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 +======TAE buffer======
 +TAE buffer is Tris-acetate buffer containing EDTA. It is used for agarose gel electrophoreses.
 +
 +=====Preparation=====
 +====50x TAE====
 +
 +Mix the following solutes and adjust to 1L by <​chem>​H2O</​chem>​.\\ ​
 +Store this stock solution at room temperature and dillute on your using.\\ ​
 +0.5x TAE works well for agarose gel electrophoresis.\\ \\ 
 +
 +^Composition||
 +|[[Tris]]|242 g|
 +|Acetic acid|57.1 mL|
 +|[[EDTA]]・2Na (2<​chem>​H2O</​chem>​)|18.6 g|
 +
 +^Final concentration||
 +|50x TAE|2M Tris-acetate/​ 50mM EDTA|
 +|working (1x)|40 mM Tris-acetate / 1 mM EDTA|
 +
 +
 +
 +======Links======
 +
 +  *[[Tris]]
 +  *[[EDTA]]
 +  *[[Comparison between TAE and TBE]]
 +  *[[TBE buffer]]
 +
 +
 +{{tag>​Buffer Preparation}}