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TdT activity

Comparison of Taq product and Pfu product

TdT(terminal deoxynucleotidyl-transferase) add a single nucleotide to 3'-ends of a blunt-ended DNA fragment.

Reaction

Taq DNA polymerase favorably append adenine to 3'-ends to produce 3'-protruding fragment. On the other hand, Pfu polymerase does not have TdT activity, and it produces blunt-ended DNA fragment.

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