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en:tdt_activity [2013/06/09 10:10] (current)
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 +======TdT activity======
 +{{ bio:​tdt_activity.png|Comparison of Taq product and Pfu product}}
 +
 +TdT(terminal deoxynucleotidyl-transferase) add a single nucleotide to 3'​-ends of a blunt-ended DNA fragment.
 +
 +======Reaction======
 +
 +Taq DNA polymerase favorably append [[nucleobase#​purine|adenine]] to 3'​-ends to produce 3'​-protruding fragment. On the other hand, Pfu polymerase does not have TdT activity, and it produces blunt-ended DNA fragment.
 +
 +
 +
 +======Links======
 +
 +  *[[TA-cloning]]
 +  *[[T-vector]]
 +
 +