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en:te [2013/06/09 10:09] (current)
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 +======TE buffer======
 +This solution is used for stock of DNA. [[EDTA]] is strong chelator of metal ions that are cofactors of endonucleases.
 +
 +=====Preparation=====
 +
 +  -Mix two stock solution, 1M Tris-HCl and 0.5M EDTA.
 +  -Add <​chem>​H2O</​chem>​ upto 1L.
 +
 +^  stock solution ​ ^^
 +|[[Tris|1M Tris-HCl (pH8.0)]]|10 mL|
 +|[[EDTA|0.5 M EDTA (pH8.0)]]|2 mL|
 +
 +
 +=> final concentration:​ 10 mM Tris-HCl, 1 mM EDTA pH 8.0
 +
 +
 +
 +{{tag>​preparation buffer}}