BioWiki

Tetracycline

Tetracycline is an antibiotic that inhibits the binding of amino-acyl tRNA to the ribosome in the protein synthesis.

NameTetracycline
PubChem image 5280962

Resistance

Tetracycline resistance protein TetB

Preparation

  1. Solve tetracycline 1g in EtOH and adjust to 50mL.
  2. Dispense into 1mL vials and store at -20°C.
  3. Add 1mL (1vial) into the 1L medium.
Stock conc.Using conc.
20mg/mL20µg/mL

References

  1. Update on acquired tetracycline resistance genes.
    Roberts MC
    FEMS Microbiol Lett245p195-203(2005 Apr 15)
  2. Bacterial resistance to tetracycline: mechanisms, transfer, and clinical significance.
    Speer BS, Shoemaker NB, Salyers AA
    Clin Microbiol Rev5p387-99(1992 Oct)
  3. The TetR family of transcriptional repressors.
    Ramos JL, Martínez-Bueno M, Molina-Henares AJ, Terán W, Watanabe K, Zhang X, Gallegos MT, Brennan R, Tobes R
    Microbiol Mol Biol Rev69p326-56(2005 Jun)