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Tris-HCl buffer

Tris-HCl buffer is commonly used for biochemical experiments. Further information is in the buffer solution page.

Name Tris base1)
Tromethamine
MeSH 68014325
Molecular Weight 121.13504 g/mol
Molecular Formula C4H11NO3
XLogP -2.7
CAS No.77-86-1
pKa(25ºC)8.08

PubChem image 6503

Preparation

1M Tris-HCl stock solution

  1. Prepare 800 mL of base solution.
  2. Add HCl soln. according to target pH.
  3. Adjust the pH of the solution.
  4. Add H2O upto 1L.

Base

MW weight amount
Tris121.135121.1g1 mol

Acid

target pH volume amount
7.470 mL12×0.07 = 0.84 mol
7.660 mL12×0.06 = 0.72 mol
8.042 mL12×0.042 = 0.50 mol

(HCl soln: 12M, 37% w/w, :!:hazardous)

Links

1)
2-amino-2-(hydroxymethyl) propane-1,3-diol