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en:tris-hcl_buffer [2013/06/09 10:10] (current)
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 +======Tris-HCl buffer======
 +Tris-HCl buffer is commonly used for biochemical experiments. Further information is in the [[buffer solution]] page.
 +
 +
 +^Name| Tris base((2-amino-2-(hydroxymethyl) propane-1,​3-diol)) |
 +^ |Tromethamine|
 +^MeSH| [[MeSH>​68014325]] |
 +^Molecular Weight| ​ 121.13504 g/mol  |
 +^Molecular Formula| <​chem>​C4H11NO3</​chem>​ |
 +^XLogP| -2.7 |
 +^CAS No.|77-86-1|
 +^pKa(25ºC)|8.08|
 +
 +{{pubchem>​large:​6503}}
 +
 +======Preparation======
 +
 +
 +
 +=====1M Tris-HCl stock solution=====
 +
 +  -Prepare 800 mL of base solution.
 +  -Add HCl soln. according to target pH.
 +  -Adjust the pH of the solution.
 +  -Add <​chem>​H2O</​chem>​ upto 1L.
 +
 +
 +
 +**Base**
 +
 +^ ^  MW  ^  weight ​ ^  amount ​ ^
 +|Tris|121.135|121.1g|1 mol|
 +
 +
 +**Acid**
 +^target pH^  volume ​ ^  amount ​ ^
 +|7.4|70 mL|12x0.07 = 0.84 mol|
 +|7.6|60 mL|12x0.06 = 0.72 mol|
 +|8.0|42 mL|12x0.042 = 0.50 mol|
 +(HCl soln: 12M, 37% w/w, **:​!:​hazardous**)
 +
 +
 +
 +
 +
 +======Links======
 +
 +  *[[Buffer solution]]
 +  *[[Tris]]
 +  *{{amazon>​us:​0879695773}}
 +
 +
 +
 +{{tag>​en:​buffer en:​preparation}}