Aminoglycoside phosphotransferase Aminoglycoside phosphotransferase Aminoglycoside phosphotransferase transfers phosphate moiety from ATP to the neomycin structure. Cells that have this enzyme can live with antibiotics such as kanamycin, neomycin and G418. EMBL-EBI/InterPro entry * IPR002575 This entry consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include:- aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and stre… ,
Amphotericin B Amphotericin B Macrolide antifungal antibiotic produced by Streptomyces nodosus obtained from soil of the Orinoco river region of Venezuela. antibiotics
Ampicillin Ampicillin Ampicillin is an antibiotics that inhibits synthesis of cell-wall. Resistance * β-lactamase * InterPro entry Preparation * Solve sodium ampicillin 5g in or 20%EtOH aq. * Adjust the volume to 50mL. * Sterilize using filter and dispense into 1mL vials. * Store at -20°C. * On using, add 1mL (1vial) into 1L medium. ,
Antibiotic G418 sulfate Antibiotic G418 sulfate Links * Antibiotics * G418 antibiotics salt ,
Antibiotics <select Language> bio:抗生物質|日本語 bio:en:Antibiotics|English </select> Antibiotics Antibiotics are used for screening of cloned cells and maintaining cells. Cell-wall synthesis inhibitors * Penicillin * Ampicillin Protein synthesis inhibitors * Kanamycin * Neomycin
Bleomycin <select Language> bio:ブレオマイシン|日本語 bio:en:Bleomycin|English </select> Bleomycin Bleomycin is an antineoplastic found by Japanese scientist Hamao Umezawa in 1966. It is produced by Streptomyces verticillus with non-ribosomal peptide synthesis. It is suggested that it chelates metal ions to activate molecular oxygen to produce free radical that breaks DNA and other molecules in the cell.
Chloramphenicol Chloramphenicol Chloramphenicol is an antibiotic that inhibits translation of protein from mRNA. It binds A site in a ribosome 50S subunit and inhibits peptidyltransferase activity. Resistance * Chloramphenicol acetyltransferase * IPR001707 Preparation * Solve chloramphenicol 1.5g in methanol and adjust to 50mL. ,
G418 (Geneticin) <select Language> bio:G418|日本語 bio:en:G418|English </select> G418 (Geneticin) G418 is an inhibitor of protein synthesis. Molecular Weight 496.553 g/mol Molecular Formula Resistance * Neor/Kanr (Aminoglycoside phosphotransferase) * InterPro entry Preparation G418 is available either solid or aqueous solution (50mg/mL) ,
Hygromycin <select Language> bio:ハイグロマイシン|日本語 bio:en:hygromycin|English </select> Hygromycin Hygromycin is an antibiotic produced by Streptomyces hygroscopicus It prohibits growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells.
Kanamycin (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2R,3S,4R,6S)-4,6-diamino-3-[(2S,3R, 4S,5S,6R)-4-amino-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2-hydroxycyc lohexyl]oxyoxane-3,4,5-triol ,
Lysozyme <select Language> bio:リゾチーム|日本語 bio:en:Lysozyme|English </select> Lysozyme Preparation 5 mg/mL Lysozyme soln. * Solve 50 mg of lysozyme in * Add upto 10 mL. * Dispense into 1 mL tubes. * Store at -20 °C :!: Repetitive melting and freezing degrades the activity of lysozyme. , ,
Neomycin Neomycin A component of NEOMYCIN that is produced by Streptomyces fradiae. On hydrolysis it yields neamine and neobiosamine B. (From Merck Index, 11th ed) (MeSH:68005601) Resistance * Aminoglycoside phosphotransferase(Neor/Kanr) * IPR012149 Links * Antibiotics * Kanamycin * G418 antibiotics
Penicillin <select Language> bio:ペニシリン|日本語 bio:en:Penicillin|English </select> Penicillin Penicillin G A penicillin derivative commonly used in the form of its sodium or potassium salts in the treatment of a variety of infections. It is effective against most gram-positive bacteria and against gram-negative cocci. It has also been used as an experimental convulsant because of its actions on GAMMA-AMINOBUTYRIC ACID mediated synaptic transmission. ,
Streptomycin Streptomycin An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis. (MeSH) Preparation * Solve Streptomycin sulfate 500mg in or 20% EtOH aq. * Adjust the volume to 50mL. * Sterilize using filter and dispense into 1mL vials. ,
Tetracycline Tetracycline Tetracycline is an antibiotic that inhibits the binding of amino-acyl tRNA to the ribosome in the protein synthesis. Resistance Tetracycline resistance protein TetB * TetB (EMBL-EBI/InterPro entry) Preparation * Solve tetracycline 1g in EtOH and adjust to 50mL.