Restriction enzyme
| ページ | 内容 | タグ |
|---|---|---|
| AatII | AatII Recognition [GACGTC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky | restriction enzyme, 3 sticky |
| AccI | AccI Recognition [GTMKAC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X * GTATAC / GTCGAC * M,K Links * Restriction enzyme * HincII * SalI * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky | restriction enzyme, 5 sticky |
| AluI | AluI Recognition [AGCT] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| ApaI | ApaI Recognition [GGGCCC] Temp. 25 ºC BufferLowMidHighAcKCl ≥75% O X X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky not_37_c_reaction | restriction enzyme, 3 sticky, not 37 c reaction |
| AvaI | AvaI Recognition [CYCGRG] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X * GTCGAG / CCCGGG * R,Y Links * Restriction enzyme * SmaI * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky | restriction enzyme, 5 sticky |
| AvaII | AvaII Recognition [GGWCC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X * GGACC / GGTCC * W Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky | restriction enzyme, 5 sticky |
| BamHI | BamHI Recognition [GGATCC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X X X Links * Restriction enzyme * M.BamHI * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky | restriction enzyme, 5 sticky |
| BsmI | BsmI Recognition [GAATGCN] Temp. 65 ºC BufferLowMidHighAcKCl ≥75% O O O X X * Origin:Bacillus stearothermophilus NUB36 Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky not_37_c_reaction | restriction enzyme, 3 sticky, not 37 c reaction |
| BstEII | BstEII Recognition [GTNACC] Temp. 60 ºC BufferLowMidHighAcKCl ≥75% X X X O X * Nucleotide N * Origin:Bacillus stearothermophilus ET Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky not_37_c_reaction | restriction enzyme, 5 sticky, not 37 c reaction |
| DpnI | DpnI DpnI cuts sites 'GATC' if the adenine is methylated. Recognition [GATC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X Reaction DpnI cuts sites 'GmATC' (mA: N6-methyladenine). The structures below are part of dam-methylated pUC19 and non-methylated pUC19, respectively. | restriction enzyme, blunt |
| DraI | DraI Recognition [TTTAAA] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| HaeII | HaeII Recognition [RGCGCY] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X * GGCGCC/AGCGCT * Nucleotide M,K Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky | restriction enzyme, 3 sticky |
| HhaI | HhaI Recognition [GCGC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X O O O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky | restriction enzyme, 3 sticky |
| HincII | HincII Recognition [GTCGAC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% reactive X O O O X Links * Restriction enzyme * SalI * AccI * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| HindIII | HindIII Recognition [AAGCTT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X O X X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky | restriction enzyme, 5 sticky |
| KpnI | KpnI Recognition [GGTACC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O X X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky | restriction enzyme, 3 sticky |
| MboI | MboI Recognition [GATC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O O O O Isoscizomer Sau3AI Sau3AI is an isoscizomer of MboI. Sau3AI can cut methylated sites, so it is often used for partial digestion of genome DNA. Links * Restriction enzyme * Sau3AI * DpnI * | restriction enzyme, 5 sticky |
| MspA1I | MspA1I Recognition [CMGCKG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X X O X * CAGCTG / CCGCGG * Nucleotide M,K Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| NarI | NarI Recognition [GGCGCC] Temp 37 °C BufferLowMidHighAcKCl ≥75% O X X X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky | restriction enzyme, 5 sticky |
| NdeI | NdeI Recognition [CATATG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X O O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky | restriction enzyme, 5 sticky |
| NsiI | NsiI Recognition [ATGCAT] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X O X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky | restriction enzyme, 3 sticky |
| PstI | PstI Recognition [CTGCAG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X O X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky | restriction enzyme, 3 sticky |
| PvuI | PvuI Recognition [CGATCG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X O X O with BSA Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky | restriction enzyme, 3 sticky |
| PvuII | PvuII Recognition [CAGCTG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O O O X O Links * Restriction enzyme * AluI * MspA1I * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| RsaI | RsaI Recognition [GTAC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O O X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| SacI | SacI Recognition [GAGCTC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O X X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky | restriction enzyme, 3 sticky |
| SalI | SalI Recognition [GTCGAC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% reactive X X O X X Links * Restriction enzyme * HincII * AccI * TaqI * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky | restriction enzyme, 5 sticky |
| Sau3AI | Sau3AI Recognition [GATC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% O O O O X Isoscizomer The recognition sites of isoscizomer, MboI will be blocked by dam methylase. On the other hand, Sau3A can cut the GATC sites even if the adenines are methylated. Therefore, Sau3AI is used for mapping or construction of library of methylated genome. | restriction enzyme, 5 sticky |
| ScaI | ScaI Recognition [AGTACT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% o o O X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| SmaI | SmaI Recognition [CCCGGG] Temp. 25 ºC BufferLowMidHighAcKCl ≥75% X O O O X Links * Restriction enzyme * AvaI * Buffer for restriction enzymes * REBASE restriction_enzyme blunt not_37_c_reaction | restriction enzyme, blunt, not 37 c reaction |
| SphI | SphI Recognition [GCATGC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O O X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky | restriction enzyme, 3 sticky |
| SspI | SspI Recognition [AATATT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X o X X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| Star activity | Star activity It has been demonstrated that under extreme non-standard conditions, restriction endonucleases are capable of cleaving sequences which are similar but not identical to their defined recognition sequence. This altered or relaxed specificity has been termed “star” activity. | restriction enzyme |
| StuI | StuI Recognition [AGGCCT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% o O X o X Links * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| TaqI | TaqI Recognition [TCGA] Temperature 65 ºC BufferLowMidHighAcKCl ≥75% X X O O X * Origin: Thermus aquaticus YTI Links * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky not_37_c_reaction | restriction enzyme, 5 sticky, not 37 c reaction |
| XbaI | XbaI Recognition [TCTAGA] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X O X X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky | restriction enzyme, 5 sticky |
最終更新: 2013/01/31 14:38
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